In the dynamic realm of drug discovery, the imperative for rapid, reliable, and scalable data acquisition is more pronounced than ever. For over two decades, Biolayer Interferometry (BLI) systems from Octet® have been instrumental in this endeavor, providing real-time, label-free interaction analysis devoid of complex fluidic systems. This article delves into how Sartorius is propelling BLI technology forward with its latest innovation, the Octet® R8e, a system poised to redefine performance standards in biomolecular studies.
Sartorius has unveiled its most sophisticated and adaptable BLI system to date, the Octet® R8e, engineered for high-throughput analysis of intricate biomolecular interactions, encompassing protein-protein and protein-small molecule engagements. Deirdre Raduns, the Manager of Product Management for Protein Analytics at Sartorius, highlighted that the R8e builds upon the established advantages of Octet® platforms—such as speed and operational simplicity—while introducing a significant enhancement in detection capabilities. This advancement allows researchers to confidently explore lower detection limits, where other BLI technologies typically falter, thereby delivering sharper data and more dependable insights for challenging applications involving small molecules, low-affinity binders, or targets present in minimal quantities.
The Octet® R8e stands as Sartorius's flagship BLI offering, catering to laboratories that demand both exceptional performance and operational flexibility. While the existing Octet® portfolio addresses a diverse range of throughput requirements, the R8e distinguishes itself with superior sensitivity and adaptable assay design, all while preserving the signature fluidics-free and user-friendly experience. This makes it a compelling choice for research teams seeking elevated data quality without the burdens of prolonged sample loading times or the intensive maintenance associated with more intricate instrumentation.
A pivotal feature of the R8e is its ability to expand the scope of BLI applications by providing near-Surface Plasmon Resonance (SPR) sensitivity. This breakthrough empowers researchers to detect and quantify interactions that were previously unattainable with BLI systems, particularly when dealing with low-molecular-weight compounds or analytes exhibiting weak binding affinities. From an operational standpoint, the instrument supports both 96- and 384-well formats, accommodating varied throughput demands. Furthermore, the inclusion of intelligent evaporation control for the 96-well format ensures sample integrity during extended, overnight experimental runs, a significant advantage for rigorous, prolonged studies.
Addressing the growing demand for automation in high-throughput laboratory environments, Octet® BLI systems are inherently designed for walk-away operation. For facilities aiming for comprehensive robotic integration, Sartorius offers the RH series (RH16 and RH96) and has forged a partnership with BIOSERO to facilitate seamless system integration and process optimization. This commitment to automation translates into heightened consistency, improved reproducibility, and more valuable time for scientific teams to dedicate to core research activities.
With thousands of Octet® BLI systems deployed globally across pharmaceutical, biotechnology, and contract research organizations, and their successful application in numerous investigational new drug submissions, these systems have garnered widespread trust. The introduction of the R8e signifies Sartorius's dedication to equipping the scientific community with advanced tools that meet the evolving challenges of biologics development, fostering innovation and enabling researchers to push the boundaries of discovery and manufacturing.